By Mark A. Strege, Avinash L. Lagu
Specialist educational and pharmaceutical researchers describe their top capillary electrophoresis recommendations for protein and peptide research. The authors current state of the art strategies for either capillary coatings and analytic detection through laser-induced fluorescence, for the advance and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis within the assessment of protein binding, together with using protein cost ladders. parts of distinct curiosity coated contain combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to accomplish proteomic reviews and functions within the box of protein-ligand binding.
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Extra info for Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology 276)
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In practice, however, a small fraction of the labeled molecules is injected into the capillary and the rest of the labeled analyte is wasted. A postcolumn labeling reaction is compatible with a sheath flow designs. An on-column “T” has been constructed to introduce labeling reagent for a postcolumn detector during the separation (23–25). The major drawback of a postcolumn labeling reaction is the slow reaction rate, which limits the detection limits. On-column labeling drastically reduces the volume of required analytes and reagents.