Download e-book for iPad: Capillary Electrophoresis of Proteins and Peptides (Methods by Mark A. Strege, Avinash L. Lagu

By Mark A. Strege, Avinash L. Lagu

Specialist educational and pharmaceutical researchers describe their top capillary electrophoresis recommendations for protein and peptide research. The authors current state of the art strategies for either capillary coatings and analytic detection through laser-induced fluorescence, for the advance and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis within the assessment of protein binding, together with using protein cost ladders. parts of distinct curiosity coated contain combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to accomplish proteomic reviews and functions within the box of protein-ligand binding.

Show description

Read or Download Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology 276) PDF

Similar biology books

New PDF release: The Biology of Seeds: Recent Research Advances

This booklet offers edited and revised papers from the 7th foreign Workshop on Seeds, held in Salamanca, Spain, in may well 2002. the main issues addressed comprise seed improvement, germination and dormancy, in addition to desiccation, seed ecology and seed biotechnology.

New PDF release: Root Hairs: Cell and Molecular Biology

Root hairs are tip-growing cells that originate from epidennal cells known as trichoblasts. Their position might be easily regarded as extending the skin region of the foundation to facilitate absorption of nutrition and water. even though, as you can see during this e-book, the basis hair is much greater than that. To an more and more better variety of plant biologists, the foundation hair is a version mobilephone.

Advances in Enzymology and Related Areas of Molecular by F. F. Nord PDF

Advances in Enzymology and comparable parts of Molecular Biology is a seminal sequence within the box of biochemistry, delivering researchers entry to authoritative reports of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unmatched view of the ancient improvement of enzymology.

Extra info for Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology 276)

Sample text

Righetti P. , and Chiari, M. (1994) Molecular modeling of acrylamide derivatives: the case of N-acryloylaminoethoxyethanol versus acrylamide and trisacryl. Electrophoresis 15, 1104–1111. 15. , and Righetti, P. G. (1996) Novel acrylamido monomers with higher hydrophilicity and improved hydrolytic stability: I. Synthetic route and product characterization. Electrophoresis 17, 723–737. 16. G. (1997) Simplified mathematical model of irreversible sample adsorption in capillary zone electrophoresis.

69, 3015–3021. 22. 22 Zhao J. , Waldron K. , Miller J. , Harke H. , and Dovichi N. J. (1992) Attachment of a single fluorescent label to peptides for determination by capillary zone electrophoresis. J. Chromatogr. 608, 239–242. 23. , and Suzuki O. (1988) Post-column detection for capillary zone electrophoresis. J. Chromatogr. 456, 375–381. 24. Roze D. J. and Jorgenson J. W. (1988) Post-capillary fluorescence detection in 24 capillary zone electrophoresis using o-Phthaldialdehyde. J. Chromatogr.

In practice, however, a small fraction of the labeled molecules is injected into the capillary and the rest of the labeled analyte is wasted. A postcolumn labeling reaction is compatible with a sheath flow designs. An on-column “T” has been constructed to introduce labeling reagent for a postcolumn detector during the separation (23–25). The major drawback of a postcolumn labeling reaction is the slow reaction rate, which limits the detection limits. On-column labeling drastically reduces the volume of required analytes and reagents.

Download PDF sample

Rated 4.54 of 5 – based on 28 votes