By Chenchen Wang, Xueping Fang, Cheng S. Lee (auth.), Nicola Volpi, Francesca Maccari (eds.)
Capillary electrophoresis (CE) is a comparatively new separation strategy compatible for dealing with small quantities of pattern vitally important in bioanalytical study and in numerous scientific, diagnostic, genetic, and forensic purposes. In Capillary Electrophoresis of Biomolecules: tools and Protocols, professional researchers within the box supply key ideas to enquire CE targeting easy and intricate carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. besides functional systems, studies discussing CE purposes on the topic of bio(macro)molecules also are integrated. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols, and key tips about troubleshooting and warding off identified pitfalls.
Authoritative and sensible, Capillary Electrophoresis of Biomolecules: equipment and Protocols offers the reader with the most recent holiday throughs and enhancements in CE and CE options utilized to a number of periods of bio(macro)molecules.
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Additional resources for Capillary Electrophoresis of Biomolecules: Methods and Protocols
Notes 1. While there is much uncertainty within the literature about improvement in peak height, area, efficiency, and resolution, there appears to be some consensus regarding an improved repeatability of the method with a water injection most likely due to the water injection “rinsing” the capillary and electrolyte before entering the sample vial and thus reducing cross contamination of the sample. 2. As the current increases very rapidly towards the end of matrix removal, it is difficult to precisely control this transition.
The use of CE/MS has been demonstrated for this purpose (25). The authors released sialic acids from glycoconjugates using mild acid hydrolysis. 5. The sheath liquid was water/methanol/ammonium acetate and the separation potential was 30 kV. Peaks corresponding to Neu5Ac and Neu5Gc were well separated. The results showed that it was possible to detect Neu5Ac released from human serum in a 9 min CE/MS run. 5. CE/MS of Glycopeptides Analysis of glycopeptides provides information on the glycan composition and the peptide to which glycans are attached.
Anal Chem 82:6504–6511 35. Auriola S, Thibault P, Sadovskaya I, Altman E (1998) Enhancement of sample loadings for the analysis of oligosaccharides isolated from Pseudomonas aeruginosa using transient isotachophoresis and capillary zone electrophoresis—electrospray—mass spectrometry. Electrophoresis 19:2665–2676 36. Aebersold R, Morrison HD (1990) Analysis of Dilute Peptide Samples by Capillary Zone Electrophoresis. J Chromatogr 516:79–88 37. Britz-Mckibbin P, Bebault GM, Chen DDY (2000) Velocity-difference induced focusing of nucleotides in capillary electrophoresis with a dynamic pH junction.