New PDF release: Biochemistry and Genetics, 2007, p.514

By Golder Wilson

This exceptional attempt prep advisor lets you try out your wisdom of crucial biochemistry and genetics techniques for the USMLE Step 1; perform with 500 USMLE Step 1-style questions with referenced solutions; assessment reasons for correct and unsuitable solutions; and construct self belief, abilities, and information.

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The reciprocal Lineweaver-Burk plot constructed by measuring enzyme velocities with and without inhibitor distinguishes competitive inhibition from noncompetitive inhibition (Fig. 10). A competitive inhibitor will have similar structure to the substrate and compete for enzyme catalytic sites, changing the effective Km but not the Vmax (Fig. 10A). A simple noncompetitive inhibitor does not resemble substrate in structure and will not compete for its binding at the enzyme catalytic site—thus changing Vmax but not Km (Fig.

3%) Type V (2%) M- McArdle syndrome (myophosphorylase deficiency—232300) Cause of Disorder Characteristics Deficiency of glucose-6phosphatase catalytic subunit (a), microsomal glucose-6phosphatase (b) or phosphate (c) transport Deficiency of lysosomal glucosidase (acid maltase) Absence of debranching enzyme Liver cells and renal tubule cells loaded with glycogen. Hypoglycemia, lacticacidemia, ketosis, hyperlipemia Fatal, accumulation of glycogen in Iysosomes, heart failure. Accumulation of branched polysaccharide Absence of branching enzyme Accumulation of unbranched polysaccharide.

High-Yield Facts 25 • Glycogenesis utilizes high energy uridine diphosphate glucose (UDPGlc) formed from glucose-1-phosphate and UTP, forming 1 → 4 links with a synthase and 1 → 6 links with branching enzymes. • Glycogen synthase adds glucose units to preexisting glucose chains on the primer protein glycogenin; the primer glucose chain is attached to the hydroxyl of a tyrosine residue. • Glycogenolysis is very different from glycogen synthesis, requiring multiple enzymes such as glycogen phosphorylase that employs phosphate to hydrolyze 1 → 4 links and debranching enzymes that hydrolyze 1 → 6 links.

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