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Additional info for Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242)
Among these, one can find deflection set point (in contact mode), oscillation amplitude and dampening (in AC modes), feedback gain (sometimes separated into a proportional gain setting and integral-derivative setting), low pass filters, scan speed, and so on. The setting of these parameters is a trial-and-error process. Each time a new sample is put into the microscope, the best values must be searched and during the process many artifacts can be produced in images. Soft samples generally must be imaged at low scan speeds and low interaction forces, otherwise glitches in the scan direction or even sample deformation may occur.
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15. Hansen, K. , Ji, H. , Wu, G. , et al. (2001) Cantilever-based optical deflection assay for discrimination of DNA single-nucleotide mismatches. Anal. Chem. 73, 1567–1571. 16. Wu, G. , Datar, R. , Hansen, K. , Cote, R. , and Majumdar, A. (2001) Bioassay of prostate-specific antigen (PSA) using microcantilevers. Nat. Biotech. 19, 856–860. 17. , O’Connor, G. , et al. (2001) Characterisation of an antibody coated microcantilever as a potential immuno-based biosensor. Biosens. Bioelectron. 17, 201–207.