Atomic Force Microscopy: Biomedical Methods and Applications by Davide Ricci, Pier Carlo Braga PDF

By Davide Ricci, Pier Carlo Braga

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Among these, one can find deflection set point (in contact mode), oscillation amplitude and dampening (in AC modes), feedback gain (sometimes separated into a proportional gain setting and integral-derivative setting), low pass filters, scan speed, and so on. The setting of these parameters is a trial-and-error process. Each time a new sample is put into the microscope, the best values must be searched and during the process many artifacts can be produced in images. Soft samples generally must be imaged at low scan speeds and low interaction forces, otherwise glitches in the scan direction or even sample deformation may occur.

Sci. 23,165–174. 19. Wu, H. , and Moy, V. T. (1998) Mechanical properties of L929 cells measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking. Scanning 20, 389–397. 20. Kuznetsov, Y. , Malkin, A. , and McPherson, A. (1997) Atomic force microscopy studies of living cells: Visualization of motility, division, aggregation, transformation and apoptosis. J. Struct. Biol. 120,180–191. 21. Wu, H. , and Moy, V. T. (1998) Mechanical properties of L929 cells measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking.

15. Hansen, K. , Ji, H. , Wu, G. , et al. (2001) Cantilever-based optical deflection assay for discrimination of DNA single-nucleotide mismatches. Anal. Chem. 73, 1567–1571. 16. Wu, G. , Datar, R. , Hansen, K. , Cote, R. , and Majumdar, A. (2001) Bioassay of prostate-specific antigen (PSA) using microcantilevers. Nat. Biotech. 19, 856–860. 17. , O’Connor, G. , et al. (2001) Characterisation of an antibody coated microcantilever as a potential immuno-based biosensor. Biosens. Bioelectron. 17, 201–207.

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